NUMERO: #20231213

Título: DNA CONTENT AND CLINICOPATHOLOGICAL FEATURES AID IN DISTINGUISHING AMELOBLASTIC CARCINOMA FROM AMELOBLASTOMA

Nome do Apresentador: Paulo Victor Mendes PENAFORT

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Objective: To evaluate DNA content by flow cytometry and Ki-67 index in AB and AC. Study design: Cases of AB and AC were retrospectively recovered. The paraffin blocks of the tumors were selected to obtain sections for the immunohistochemical reactions and preparation of the cell suspension for acquisition in a flow cytometer. The Random Forest package of the R software was used to verify the importance of each variable for the diagnosis. Results: 32 cases of AB and 05 of AC were included in this study. The differences in Ki-67 labeling rates were not statistically significant. A higher fraction of cells in 2c (G1) was correlated with the diagnosis of AB while higher rates of 5cER were correlated with AC. The determination of cell percentage thresholds in 2c and 5cER for the predicted outcomes (AB and AC), through multiple logistic regression, showed a cut-off of <63.5% in 2c and >4.2% in 5cER favorable to high risk. The random forest model highlighted histopathological findings and parameters of DNA ploidy study as important features for distinguishing AB from AC. Conclusion: Our findings suggest that the DNA content in tumor cells can be an ancillary tool in the diagnosis of AB and AC.

Autor 1:  Paulo Victor Mendes PENAFORT

E-mail 1:  [email protected]

Autor 2:   André Caroli ROCHA

E-mail 2:  [email protected]

Autor 3 :  Fernanda Viviane MARIANO

E-mail 3:  [email protected]

Autor 4:  Jean Nunes dos SANTOS

E-mail 4:  [email protected]

Autor 5:  Márcio Campos OLIVEIRA

E-mail 5:  [email protected]

Autor 6:  Pablo Agustin VARGAS

E-mail 6:  [email protected]

Autor 7:  Marcelo SPERANDIO

E-mail 7:  [email protected]