NUMERO: #20231128

Título: VALIDATION OF METHOD TO EVALUATE ORAL DISORDERS BIOMARKERS IN ORAL EXFOLIATED CELLS

Nome do Apresentador: Lukas Mendes de ABREU

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Áreas afins no caráter multidisciplinar

Resumo: Objective: We aimed to describe and validate a protocol for measuring intracellular content of reactive oxygen species (ROS) and glutathione (GSH) in exfoliated oral cells. Study Design: Oral exfoliated cells from eight healthy volunteers were collected and washed by centrifugation in PBS. The cell pellet of each volunteer was divided into two equal parts. One part was immediately stained and the other was stimulated with 5µM hydrogen peroxide (H2O2) for 1 hour at 37º C to generate oxidative stress (OS). Cells were stained with the fluorescent probes 6-carboxy-2´ diacetate, 7´ dichlorodihydrofluorescein (H2DCFDA; ROS) and ThiolTrackerT Violet (Glutathione Detection Reagent; GSH), as recommended by the manufacturer. Cells were mounted on glass slides and immediately evaluated under microscopic equipped with epifluorescence. The average value of the expression from 100 cells from each volunteer was presented as arbitrary fluorescence units (AFU). Results: In oral cells exposed to H2O2, intracellular expression of ROS was increased compared to cells not exposed (3.851 vs 1.106; p<0.0338) while GSH was decreased (9.011 vs 15.901; p<0.0289). Conclusion: This protocol proved to be effective for oxidative stress biomarkers in oral exfoliated cells, and could be used in new clinical studies.

Autor 1:  Lukas Mendes de ABREU

E-mail 1:  [email protected]

Autor 2:   Cíntia Rodrigues da SILVA

E-mail 2:  [email protected]

Autor 3 :  Ana Laura Ferreira BORTOLETO

E-mail 3:  [email protected]

Autor 4:  Giovana Barros NUNES

E-mail 4:  [email protected]

Autor 5:  Gisele Zoccal MINGOTI

E-mail 5:  [email protected]