NUMERO: #20231121

Título: CEPHAELINE REPRESENTS A POTENTIAL THERAPEUTIC AGENT FOR ORAL SQUAMOUS CELL CARCINOMA

Nome do Apresentador: Amanda Almeida LEITE

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Oncologia

Resumo: Objective: Cancer stem cells (CSCs) are commonly related to tumor recurrence being responsible for poor overall survival rates in oral squamous cell carcinoma (OSCC). In the present study, we evaluated the anticancer properties of cephaeline in OSCC cell lines, including OSCC-CSC regulation. Study design: SCC4, SCC9, and SCC25 were used as in vitro OSCC models to assess the cephaeline effects. Cell viability was determined by MTT assay, and CSCs content was evaluated by tumorsphere functional assay. The histone acetylation (H3K9ac) status and NFκB signaling were determined by immunofluorescence staining. Results: A single dose administration of cephaeline disrupted tumorspheres formation, while the number of tumorspheres was not affected by cisplatin treatment. Also, we found that cephaeline inhibited the NFκB pathway and increased acetylation levels of H3 histone. Finally, cephaeline was able to sensitize OSCC cells to cisplatin. Conclusion: These findings suggest that cephaeline is a potential therapeutic agent for OSCC by disrupting tumorspheres. In addition, the combination of cephaeline and cisplatin might be a promising treatment for oral cancer. In vivo studies are needed to validate these findings.

Autor 1:  Amanda Almeida LEITE

E-mail 1:  [email protected]

Autor 2:   Luan César da SILVA

E-mail 2:  [email protected]

Autor 3 :  Gabriell Bonifácio BORGATO

E-mail 3:  [email protected]

Autor 4:  Alan Roger SANTOS-SILVA

E-mail 4:  [email protected]

Autor 5:  Márcio Ajudarte LOPES

E-mail 5:  [email protected]

Autor 6:  Rogerio Moraes CASTILHO

E-mail 6:  [email protected]

Autor 7:  Pablo Agustin VARGAS

E-mail 7:  [email protected]