NUMERO: #20231036

Título: COMPARATIVE STUDY OF SALIVA AND SERUM CYTOKINE CONCENTRATIONS IN KIDNEY TRANSPLANT PATIENTS

Nome do Apresentador: Fabiana MARTINS

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Objective: Recent studies report that kidney recipients exhibit poor outcomes associated with increased levels of pro-inflammatory cytokines and viral infections. This study aimed to analyze and compare the feasibility of using saliva to measure inflammatory cytokines and viremia in kidney recipients. Study design: Saliva and blood samples were collected from 48 patients. For herpesviruses detection, a Pan-Herpes PCR was used, and for the cytokines (IFNy, IL-10, IL-4, IL-6, IL-8, and TNFa) profile, a multiplex kit. The accuracy between the herpesvirus detection, using different fluids. Statistically analyzes included Bowker symmetry and Pearson's test. Results: HSV-PAN PCR detection accordance between results was 53.9% and VZV-PAN PCR 32.6%. No symmetry was observed, with a higher tendency in positive cases in saliva. The cytokines levels results presented that the correlations between the two types of samples were very weak or weak (>0.39). The exceptions included concentrations for IFNy within the range of moderate correlation, and TNFa significant (p-value < 0.05). Conclusion: Salivary herpesviruses detection demonstrates the intense viral excretion observed in transplanted patients, while in the study of cytokines, positive correlation of TNFa expression in serum and salivary suggests its importance in the local and systemic inflammatory response in this group of patients.
São Paulo Research Foundation (FAPESP), grant # 2021/07490-0; 2018/02568-8

Autor 1:  Fabiana MARTINS

E-mail 1:  [email protected]

Autor 2:   Debora Macedo

E-mail 2:  [email protected]

Autor 3 :  Stephanie Anasenko

E-mail 3:  [email protected]

Autor 4:  Rodrigo Zerbinati

E-mail 4:  [email protected]

Autor 5:  Fabiana Agena

E-mail 5:  [email protected]

Autor 6:  Ligia Pierrotti

E-mail 6:  [email protected]

Autor 7:  Paulo Henrique Braz-Silva

E-mail 7:  [email protected]