NUMERO: #20231027

Título: INFLUENCE OF DIFFERENT FIXATIVES, TIME AND TEMPERATURE ON THE QUALITY OF HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL REACTIONS

Nome do Apresentador: Stella Graf PAES

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Fixation of biopsied materials is a critical step in histological and immunohistochemical processing. However, oral pathology labs often receive improperly collected materials. Objective: This study evaluated the effect of different solutions stored at various temperatures on immunostaining in immunohistochemical reactions. Study Design: Skin from the backs of euthanized Wistar rats was collected using an 8 mm punch pen and placed in different solutions, including buffered and unbuffered formaldehyde, alcohol, and saline, for 24 hours, 7 days, and 30 days. The specimens were then stained with hematoxylin and eosin and the Monoclonal Mouse Anti-Human Muscle Actin antibody (Code HHF-35, Dako Denmark). Results: Histological analysis revealed that specimens fixed in formalin at room temperature had higher immunostaining and more homogeneous tissue structures than specimens stored in the refrigerator. Fragments stored in alcohol and saline had reduced immunostaining and tissue autolysis, respectively. Conclusion: The study concludes that rat skin samples fixed with formalin at room temperature produce better histological and immunohistochemical results. Storage in the refrigerator resulted in a higher incidence of tissue artifacts.

Undergraduate research scholarship CNPq/PIBIC

Autor 1:  Stella Graf PAES

E-mail 1:  [email protected]

Autor 2:   Maria Luiza Granghelli FERREIRA

E-mail 2:  [email protected]

Autor 3 :  Larissa Araujo AGATTI

E-mail 3:  [email protected]

Autor 4:  Marcelo SPERANDIO

E-mail 4:  [email protected]

Autor 5:  Isadora Ferreira BARBOSA

E-mail 5:  [email protected]

Autor 6:  Paulo de Camargo MORAES

E-mail 6:  [email protected]

Autor 7:  Victor MONTALLI

E-mail 7:  [email protected]