NUMERO: #20230872

Título: NONINVASIVE LIQUID BIOPSY OF TEAR FLUIDS MAY REFLECT ORAL CANCER INITIATION

Nome do Apresentador: Erison Santana Dos SANTOS

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Oncologia

Resumo: Objective: We aimed to find biomarkers of progression from oral leukoplakias (OL) to oral squamous cell carcinoma (OSCC) using the noninvasive liquid biopsy of tear fluids. Study Design: Unstimulated tears from patients with OL (N=10), proliferative verrucous leukoplakia (PVL) (N=10), OSCC (n=14), and control patients (N=10) were collected and submitted to discovery-based proteomics by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The data were analyzed by the software MaxQuant (proteomics), Byonics (N-glycoproteomics), and PEAKS database (post-translational modifications) (ANOVA following the Tukey test assuming p<0.05). Proteomic data were integrated with analysis of public databases to validate the findings. Results: We identified a panel of proteins with differential abundance among the groups that present the potential capacity to predict malignant transformation. Many proteins are correlated with clinicopathological features, such as epithelial dysplasia, lymph node metastasis, and clinical stages of the disease. In addition, using public databases we demonstrated that some proteins identified in our panel of biomarkers are druggable and may be useful as therapeutic targets. Conclusion: Liquid biopsy from tear fluids may be a powerful noninvasive tool to provide insights into the biology of OSCC.

Financial Support: FAPESP 22/04490-1.

Autor 1:  Erison Santana Dos SANTOS

E-mail 1:  [email protected]

Autor 2:   Daniela Campos GRANATO

E-mail 2:  [email protected]

Autor 3 :  Carolina Moretto CARNIELLI

E-mail 3:  [email protected]

Autor 4:  Ana Carolina PRADO-RIBEIRO

E-mail 4:  [email protected]

Autor 5:  Alan Roger SANTOS-SILVA

E-mail 5:  [email protected]

Autor 6:  Marcio Ajudarte LOPES

E-mail 6:  [email protected]

Autor 7:  Adriana Franco PAES LEME

E-mail 7:  [email protected]