NUMERO: #20230559

Título: INFLUENCE OF AXL RECEPTOR IN ORAL SQUAMOUS CELL CARCINOMA (OSCC) AND ON THE ASSOCIATED PHENOTYPE OF MYELOID CELLS

Nome do Apresentador: Camyla Rodrigues NASCIMENTO

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Oncologia

Resumo: Objective: AXL is a receptor tyrosine kinase that is overexpressed and considered an oncogene in various types of solid tumors. The objective is determine the influence of AXL receptor on OSCC and the associated immune response.

Study Design: A syngeneic orthotopic model of OSCC was induced by injecting 5x10^5 MOC2 cells directly into the floor of the mouth of WT and AXL KO mice. Animals were euthanized after two weeks, when 20% of the initial body weight was lost and the tumors dissected, weighted, and measured. Spleens were collected and dissociated for flow cytometry analyses of the phenotype of myeloid cells. Splenocytes were stained with antibodies for CD11b, F4/80, CD80, MHC-II and MertK.

Results: There was no significant difference in tumor size or weight between WT and AXL KO mice. There was a trend of increased numbers of F4/80 macrophages and a significant (p=0.03) increase in CD11b+/MHCII+ cells in AXL KO mice. No difference in CD11b+CD80+ or CD11b+MertK+ cells between WT and AXL KO mice.

Conclusion: Lack of AXL receptor had no effect on experimental OSCC tumor growth. There is a shift to an M2-like phenotype of myeloid cells in AXL KO mice.

Financial Support: FAPESP #2022/01487-0; #2020/00394-2.

Autor 1:  Camyla Rodrigues NASCIMENTO

E-mail 1:  [email protected]

Autor 2:   Álvaro Formoso PELEGRIN

E-mail 2:  [email protected]

Autor 3 :  Ianny Brum REIS

E-mail 3:  [email protected]

Autor 4:  Milena Moraes de CARVALHO

E-mail 4:  [email protected]

Autor 5:  Danilo Paschoal FERRAREZI

E-mail 5:  [email protected]

Autor 6:  Aldenora Maria Ximenes RODRIGUES

E-mail 6:  [email protected]

Autor 7:  Carlos ROSSA Jr

E-mail 7:  [email protected]