NUMERO: #20230542

Título: DEGENERATIVE ALTERATIONS INDUCED BY SLEEP DEPRIVATION IN SUBLINGUAL GLANDS OR THE RATS

Nome do Apresentador: Veronica Quispe YUJRA

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Sleep is a fundamental process for the maintenance and balance of biological mechanisms therefore, the lack of it can cause or worsen health problems. We aimed to evaluate whether sleep deprivation can trigger proliferative and apoptotic processes in sublingual glands. 24 male rats were distributed into three groups: Control (n=8) the animals were not subjected to any procedure; Sleep Deprivation (n=8) the animal was submitted to paradoxical sleep deprivation for 96h; Rebound (n=8) the animals were subjected to sleep deprivation for 96h more 96h without intervention. Morphological alterations such as acinar atrophy, hypertrophy, hyperplasia, and metaplasia were investigated. Also, we evaluated the immunoexpression of proteins such as Ki-67, p16, activate Caspase-3, and BCL-2. The results showed that paradoxical sleep deprivation induced tissue degeneration from the presence of pyknosis, vacuoles, and areas of salivary retention, in both experimental groups. Expression of cleaved caspase-3 and BCL-2 increased in both sleep deprivation and rebound groups. The analysis of Ki-67 showed an increase in expression only in the rebound group, associated with a decrease in p16. Therefore, sleep deprivation can trigger a degenerative tissue process, through the dysregulation of apoptosis and proliferative activity in the sublingual glands of rats.

Autor 1:  Veronica Quispe YUJRA

E-mail 1:  [email protected]

Autor 2:   Gabriel Carvalhal de AGUIAR

E-mail 2:  [email protected]

Autor 3 :  Andrea Cristina de Moraes MALINVERNI

E-mail 3:  [email protected]

Autor 4:  Ana Carolina Flygare SOUZA

E-mail 4:  [email protected]

Autor 5:  Hanna Karen Moreira ANTUNES

E-mail 5:  [email protected]

Autor 6:  Daniel Araki RIBEIRO

E-mail 6:  [email protected]