NUMERO: #20230240

Título: IMMUNOHISTOCHEMICAL ANALYSIS OF EPITHELIAL-MESENCHYMAL TRANSITION BIOMARKERS IN ADENOID CYSTIC CARCINOMA

Nome do Apresentador: Carla Samily de Oliveira COSTA

Categoria do Trabalho: Painel de pesquisa científica (PPC)

Área Temática: Patologia Oral

Resumo: Objective: To evaluate the immunoexpression of the epithelial-mesenchymal transition biomarkers (Twist1, Snail1, Vimentin, and E-cadherin) in specimens of adenoid cystic carcinoma (ACC), and to investigate possible correlations between them.
Study Design: Twenty cases of ACC were selected for quantitative and semi-quantitative immunohistochemical analysis and by cellular compartment. To assess survival, association with clinicopathological parameters, and histopathological grading systems, the cases were categorized into low and high expression.
Results: The immunohistochemical analysis revealed significant differences between the immunoexpression of Vimentin when compared with the clinical variables: the presence of lymph node metastasis, distant metastasis, late clinical staging, and death (p<0.05). In addition, a significant difference was found between the cytoplasmic immunoexpression of Snail1 with the non-occurrence of vascular invasion (p<0.05). There were no significant differences between clinicopathological parameters and grading systems, and no significant associations between immunohistochemical variables and specific survival and disease-free survival (p>0.05). Regarding the correlation test, there was an inversely proportional correlation between the cytoplasmic expression of Twist1 and Vimentin (p<0.05).
Conclusion: In this study, it was possible to conclude that epithelial-mesenchymal transition biomarkers are significantly expressed in ACC specimens, possibly associated with their development process, thus highlighting the role of vimentin.
Financial support: CNPq and CAPES.

Autor 1:  Carla Samily de Oliveira COSTA

E-mail 1:  [email protected]

Autor 2:   Larissa Santos Amaral ROLIM

E-mail 2:  [email protected]

Autor 3 :  Rodrigo Porpino MAFRA

E-mail 3:  [email protected]

Autor 4:  Lélia Batista de SOUZA

E-mail 4:  [email protected]

Autor 5:  Cristiane Helena SQUARIZE

E-mail 5:  [email protected]

Autor 6:  Rogério Moraes CASTILHO

E-mail 6:  [email protected]

Autor 7:  Leão Pereira PINTO

E-mail 7:  [email protected]